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year 27, Issue 2 (ICOP & ICPET 2021 2021)
ICOP & ICPET _ INPC _ ICOFS 2021, 27(2): 741-745 |
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Tavassoli Koupaei A, Mostafavi Amjad J, Dehghan Niry M. Improving the accuracy of E. coli K-12 strain MG1655 biomass concentration measurement using optical density. ICOP & ICPET _ INPC _ ICOFS 2021; 27 (2) :741-745
URL: http://opsi.ir/article-1-2469-en.html
URL: http://opsi.ir/article-1-2469-en.html
1- Department of Physics, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan
Abstract: (1283 Views)
Optical density is widely used as a proxy for measuring biomass concentration in microbiology approaches when light attenuation is dominated by scattering. However, the applicability of OD-based correlations is highly dependent on the wavelength. In this study, using the optical density measurement method, we obtained the growth curve for E.coli K-12 strain MG-1655 and displayed that OD indicates the concentration of bacterial biomass solely in the visible wavelength range.
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