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Volume 26 - ICOP & ICPET 2020
ICOP & ICPET _ INPC _ ICOFS 2020, 26 - ICOP & ICPET 2020: 657-660 |
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Azizi S, Ghandi-Monfared M, Aminoroaya N, Sarabi H, Arandian A, Latifi H. Design and Implementation of a Digital Scanning Laser Light-Sheet Microscope for Fluorescence Imaging of Biological Samples. ICOP & ICPET _ INPC _ ICOFS 2020; 26 :657-660
URL: http://opsi.ir/article-1-2148-en.html
URL: http://opsi.ir/article-1-2148-en.html
Saeed Azizi * 
1, Mohammad Ghandi-Monfared1 , Neda Aminoroaya1 , Hassan Sarabi1 , Alireza Arandian1 , Hamid Latifi1
1, Mohammad Ghandi-Monfared1 , Neda Aminoroaya1 , Hassan Sarabi1 , Alireza Arandian1 , Hamid Latifi1
1- Shahid Beheshti University
Abstract: (1632 Views)
Recently, utilizing and development of light-sheet microscopy, as a new type of fluorescence imaging technique, have been noticed by vast number of researchers in biology and optics. Special advantages of this technique include ability of fast imaging, low rates of photo-bleaching and photo-toxicity and, capability of in-depth imaging of live samples like small embryos. In this work, we designed and implemented a light-sheet microscope. The light sheet was generated using a fast scanning beam of laser from one side of a sample. This mechanism facilitates exact control of the sheet quality and its uniformity. Axial and lateral resolutions of 30µm and 1µm using a laser pump of 532nm were achieved, respectively. The light-sheet height could be controlled using a laser scanner. Then, we used the microscope to test the quality of the imaging system in case of living zebra-fish embryos.
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