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Deljoor M, Mozdoor dashtabi M, Massudi R. Resolution enhancement of confocal laser scanning microscopy by detection pinhole axial modulation technique. ICOP & ICPET _ INPC. 2018; 24 :173-176
URL: http://opsi.ir/article-1-1538-en.html
1- Shahid Beheshti University, G.C., Laser and Plasma Research Institute
Abstract:   (1336 Views)
With the invention of confocal microscopy, in addition to the enhancement of resolution, 3D imaging of thick highly-scattering samples became possible. In this article, an instant data acquisition processing method is introduced using axial modulation of detection pinhole in order to increase the axial resolution of a laser scanning confocal microscope (SCM). This method, improves optical sectioning as well and also resolves the ambiguity of sample positioning (above or below the scanning plane) existing in conventional confocal microscopes.
 
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Type of Study: Research | Subject: Special

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