Recently, utilizing and development of light-sheet microscopy, as a new type of fluorescence imaging technique, have been noticed by vast number of researchers in biology and optics. Special advantages of this technique include ability of fast imaging, low rates of photo-bleaching and photo-toxicity and, capability of in-depth imaging of live samples like small embryos. In this work, we designed and implemented a light-sheet microscope. The light sheet was generated using a fast scanning beam of laser from one side of a sample. This mechanism facilitates exact control of the sheet quality and its uniformity. Axial and lateral resolutions of 30µm and 1µm using a laser pump of 532nm were achieved, respectively. The light-sheet height could be controlled using a laser scanner. Then, we used the microscope to test the quality of the imaging system in case of living zebra-fish embryos.

Keywords: Fluorescence bio-imaging, Laser scanning, Light-sheet microscopy, Optical sectioning

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