Volume 26 - ICOP & ICPET 2020                   ICOP & ICPET _ INPC 2020, 26 - ICOP & ICPET 2020: 561-564 | Back to browse issues page

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Seyed Nazari M H, Chavoshi nezhad S, Hosseini M, Karimi tar H, Dargahi L, Latifi H et al . Differentiation of Human Dental Pulp Stem Cell using Optical Stimulation of Gold Nanoparticle. ICOP & ICPET _ INPC. 2020; 26 :561-564
URL: http://opsi.ir/article-1-2120-en.html
1- Shahid Beheshti University
Abstract:   (426 Views)
In this article, the photothermal stimulation technique was used for cell differentiation of human dental pulp stem cells (hDPSCs) into nerve cells. Experimentally the hDPSCs were plated at the density of 150000 cells/ml in T25 flask and treatment with AuNPs in 10-5 ppm and 10-1 ppm. Then optical stimulation was conducted. A day after the last light stimulation, the viability of cells was analyzed with the MTT assay. The result of the MTT assay revealed significant more viability in photothermal stimulation as compared with the control group. In addition, we compared this method with the optogenetic technique using opsin-expressing hDPSCs. For the assessment of differentiation, the expression of gene Tubulin was studied. Analysis of quantitative PCR (qPCR) data shows that both technics can differentiate the stem cells from neuron cells.
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Type of Study: Research | Subject: Special

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