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Volume 26 - ICOP & ICPET 2020
ICOP & ICPET _ INPC _ ICOFS 2020, 26 - ICOP & ICPET 2020: 561-564 |
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Seyed Nazari M H, Chavoshi nezhad S, Hosseini M, Karimi tar H, Dargahi L, Latifi H et al . Differentiation of Human Dental Pulp Stem Cell using Optical Stimulation of Gold Nanoparticle. ICOP & ICPET _ INPC _ ICOFS 2020; 26 :561-564
URL: http://opsi.ir/article-1-2120-en.html
URL: http://opsi.ir/article-1-2120-en.html
Mir Hossein Seyed Nazari1 
, Sara Chavoshi nezhad1 , Mohammad Hosseini1 , Hedieh Karimi tar1 , Leila Dargahi1 , Hamid Latifi1 , Mohammad Ismail Zibaii * 1
, Sara Chavoshi nezhad1 , Mohammad Hosseini1 , Hedieh Karimi tar1 , Leila Dargahi1 , Hamid Latifi1 , Mohammad Ismail Zibaii * 1
1- Shahid Beheshti University
Abstract: (1576 Views)
In this article, the photothermal stimulation technique was used for cell differentiation of human dental pulp stem cells (hDPSCs) into nerve cells. Experimentally the hDPSCs were plated at the density of 150000 cells/ml in T25 flask and treatment with AuNPs in 10-5 ppm and 10-1 ppm. Then optical stimulation was conducted. A day after the last light stimulation, the viability of cells was analyzed with the MTT assay. The result of the MTT assay revealed significant more viability in photothermal stimulation as compared with the control group. In addition, we compared this method with the optogenetic technique using opsin-expressing hDPSCs. For the assessment of differentiation, the expression of gene Tubulin was studied. Analysis of quantitative PCR (qPCR) data shows that both technics can differentiate the stem cells from neuron cells.
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