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Latifi H, saeedian Z, Zibaii M I, Taghipour Z. Measuring the inhibination rate of growth of E. coli k-12 in the presence of silver nano particles by tapered optical fiber biosensor. ICOP & ICPET _ INPC _ ICOFS 2014; 20 :1113-1116
URL: http://opsi.ir/article-1-446-en.html
URL: http://opsi.ir/article-1-446-en.html
Abstract: (5757 Views)
In this paper the antibacterial properties of silver nanoparticles (SNPs) against the Escherichia coli (E.coli) by culturing the organisms in liquid broth in the presence different concentration of SNP was studied by optical fiber sensor. The sensor was fabricated using heat-pulling method by CO2 laser and bacteria immobilized on the optical fiber surface. Then the sensor was surrounded with different concentration of SNP. Also, due to interaction of SNP and immobilized bacteria on the optical fiber surface, changed the effective refractive index around the fiber and this affects the evanescent field leading to changes in optical throughput. The results show that E.coli growth was inhibited by different concentrations of SNP. The inhibition rate of the E. coli growth for SNP concentrations in the range of 0 to 50 μg ml-1 were measured to be from 58.0×10-2h-1 to -35.0×10-2 h-1 and from - 1.3×10-3 h-1 to - 8.88×10-3 h-1for colony counting and optical fiber biosensor, respectively. This novel sensing method, promises new application such as rapid analysis of antibacterial effects of SNPs.
Keywords: Tapered optical fiber sensor, refractive index, E. coli, inhibitation rate, Silver nanoparticles (SNPs)
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