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year 27, Issue 2 (ICOP & ICPET 2021 2021)
ICOP & ICPET _ INPC _ ICOFS 2021, 27(2): 629-633 |
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Naseri A, Khalilzadeh J, Darbani S M R, Eslami Majd A, Akbari M R, Faridfar S. Investigation of the constituent elements of escherichia coli bacterial in different growth phases using laser induced breakdown spectroscopy. ICOP & ICPET _ INPC _ ICOFS 2021; 27 (2) :629-633
URL: http://opsi.ir/article-1-2444-en.html
URL: http://opsi.ir/article-1-2444-en.html
Ahmad Naseri *1 
, Javad Khalilzadeh1 , Seyyed Mohammad Reza Darbani2 , Abdollah Eslami Majd2 , Mohammad Reza Akbari3 , Shahin Faridfar3
, Javad Khalilzadeh1 , Seyyed Mohammad Reza Darbani2 , Abdollah Eslami Majd2 , Mohammad Reza Akbari3 , Shahin Faridfar3
1- Center of Science and Technology Laser Optics, University of Emam Hossein
2- Institute of Optics and Laser Science and Technology, University of Maleke Ashtar
3- Center of Science and Technology Biology, University of Emam Hossein
2- Institute of Optics and Laser Science and Technology, University of Maleke Ashtar
3- Center of Science and Technology Biology, University of Emam Hossein
Abstract: (940 Views)
In this paper, constituent elements of Escherichia Coli bacterial were investigated and compared using Laser Induced Breakdown Spectroscopy (LIBS). The studied sample is non-pathogenic Specimen of Escherichia Coli that has been cultured in three time phases of growth phase with optical density 0.4, 0.8 and 1.2. Bacterial samples were prepared at each stage of the growth phase, after freezdry are transformed into tablets. Bacteria spectrum are obtained in all three stages of growth phase with standard layout and desktop LIBSCAN100. Based on the results of spectroscopy under the same experimental conditions, and compared with glass substrate spectrum, elements of calcium, sodium, potassium and CN molecular bonds were observed in the spectra of samples and their relative intensities were compared with each other. The results of the spectroscopy investigation show that laser Induced Breakdown Spectroscopy is capable of identification and discrimination different phases of bacterial growth from each other.
Keywords: Laser Induced Breakdown Spectroscopy, Bacteria, Growth Phases, Sample preparation, Escherichia Coli
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