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Ganjkhani Y, Ahadi Akhlaghi E, Chaharsooghi M, Moradi A. Super-resolution by Structured Illumination in Mirau Digital Holographic Microscopy
. ICOP & ICPET _ INPC _ ICOFS 2018; 24 :465-468
URL: http://opsi.ir/article-1-1557-en.html
URL: http://opsi.ir/article-1-1557-en.html
1- Department of Physics, Institute for Advanced Studies in Basic Sciences (IASBS), 45137-66731, Zanjan , Iran
2- Optics Research Center, Institute for Advanced Studies in Basic Sciences (IASBS), 45137-66731, Zanjan, Iran
2- Optics Research Center, Institute for Advanced Studies in Basic Sciences (IASBS), 45137-66731, Zanjan, Iran
Abstract: (2449 Views)
In this paper we apply structured illumination in Mirau digital holographic microscopy to improve the lateral resolution. Digital holographic microscopy (DHM) is an effective and non-contact technique for 3D imaging of phase objects. Among different DHM setups, Mirau DHM due to its simple and vibration-immune nature is of a high interest. Mirau objectives with high NA are expensive and low NA ones suffer from low lateral resolution. It has been shown that by imaging 1D gratings on the sample we can obtain super-resolution in DHM in 1 direction and to have super-resolution in all directions the gratings should be rotated. We demonstrate that by projecting 2D gratings, instead, super-resolved 3D images in all directions may be obtained. We apply the technique to resolve microparticles within aggregations of them which can never be resolved under uniform illumination of the Mirau DHM setup. The resolution power of the system was improved from 1.3 to 0.84 microns.
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