Optics and Photonics Society of Iran

In this paper we proceed with the implementation of a structured illumination microscope (SIM). SIM systems consist of two main parts i) Optical setup design and ii) image reconstruction algorithm. In the optical setup, we make use of a reflective spatial light modulator (SLM) in order to generate structured illumination. Nine different diffraction grating patterns were produced for microscopy through the SLM with three different related angles and phases. Moreover, we implemented a computer code in MATLAB based on Fourier optics to reconstruct the final image. According to our results, the fine details of a given biological sample were resolved by the SIM-SLM system due to an appreciable increase in resolution.