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ICOP & ICPET 2018, 24 - : 17-20 Back to browse issues page
Structured Illumination Microscope based on a Spatial Light Modulator for High-resolution Microscopy
Mohammad Ghandi Monfared * 1, Amir Hossein Baradaran Ghasemi 1, Hamid Latifi 1
1- Shahid Beheshti University
Abstract:   (182 Views)

In this paper we proceed with the implementation of a structured illumination microscope (SIM). SIM systems consist of two main parts i) Optical setup design and ii) image reconstruction algorithm. In the optical setup, we make use of a reflective spatial light modulator (SLM) in order to generate structured illumination. Nine different diffraction grating patterns were produced for microscopy through the SLM with three different related angles and phases. Moreover, we implemented a computer code in MATLAB based on Fourier optics to reconstruct the final image. According to our results, the fine details of a given biological sample were resolved by the SIM-SLM system due to an appreciable increase in resolution.

Keywords: Structured illumination microscopy, Super-resolution microscopy, Spatial Light Modulator
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Type of Study: Research | Subject: Special
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Ghandi Monfared M, Baradaran Ghasemi A H, Latifi H. Structured Illumination Microscope based on a Spatial Light Modulator for High-resolution Microscopy. ICOP & ICPET. 2018; 24 :17-20
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Volume 24 - Back to browse issues page
انجمن اپتیک و فوتونیک ایران Optics and Photonics Society of Iran
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