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ICOP & ICPET 2017, 23 - : 757-760 Back to browse issues page
Design and Operation a Lightguide-based Total Internal Reflection Fluorescence Microscope for Imaging of CdSe Quantum dots
Neda Rousaie Miss * 1, Fariba Japelaghi Miss, Elham Sheykhi Mrs, Noura Afshar Miss, Narges Ghalambor Miss, Mohammad Amin Bassam Dr, Sharareh Tavaddod Dr, Batool Sajad Dr
Abstract:   (985 Views)

In this works a lightguide-based total internal reflection fluorescence microscope is designed and demonstrated to detect the fluorescence of fluorophore molecules labeled in the sample. Total internal reflection fluorescence microscopy (TIRFM) is a technique that utilizes the total internal reflection phenomenon. Indeed, when total internal reflection occurs, an evanescent wave is generated in the less dense medium which is used for illuminating the sample and consequently the possibility of observing a superficial (instead of bulk) part of fluorophore-labeled sample is opened up. For this purpose, an inverted microscope first has been designed using the available optic and optomechanical devices. Subsequently, the arrangement of lightguide-based total internal reflection fluorescence microscope has been done by means of the inverted microscope to carry out the imaging operation for some CdSe quantum dot samples

Keywords: total internal reflection, fluorescence, evanescent wave, fluorophore
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Type of Study: Research | Subject: Special
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Rousaie N, Japelaghi F, Sheykhi E, Afshar N, Ghalambor N, Bassam M A, et al . Design and Operation a Lightguide-based Total Internal Reflection Fluorescence Microscope for Imaging of CdSe Quantum dots. ICOP & ICPET. 2017; 23 :757-760
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انجمن اپتیک و فوتونیک ایران Optics and Photonics Society of Iran
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