[Home ] [Archive]   [ فارسی ]  
:: Volume 23 - ::
ICOP & ICPET 2017, 23 - : 741-744 Back to browse issues page
Application of Fresnel diffraction from a transparent plate in microscopy of phase samples
Samira Ebrahimi * 1, Masoomeh Dashtdar Dr
Abstract:   (1066 Views)

In this paper, a method based on Fresnel diffraction from a parallel phase plate is presented for the phase study of micron- sized transparent samples. If a transparent plate is located in the path of a monochromatic beam of laser light, the diffraction fringes appear due to an abrupt change in the optical path length (OPL) difference between the part of the incoming light passing through the plate and the other propagating in the air. Now, if another phase sample is located in the path of the incoming light to the plate, the diffraction fringe pattern changes. the phase information of the sample can be extracted within the fringes by different fringe analysis methods. The presented setup is simple in comparison with complex interferometry techniques and the fringe pattern is stable against any mechanical and optical vibrations. Also, the accuracy of phase measurement is comparable with other common methods in which anti-vibration tables and high precision optical elements are required. By using this method, some measurements are performed on Red Blood Cells (RBC) and the results are compared with digital holographic microscopy (DHM) technique. The results indicate the high efficiency of technique in study of microscopic samples and nanometric variations in the membrane of living cells

Keywords: Fresnel diffraction, fringe analysis, living cell, phase plate.
Full-Text [PDF 597 kb]   (572 Downloads)    
Type of Study: Research | Subject: Special
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ebrahimi S, Dashtdar M. Application of Fresnel diffraction from a transparent plate in microscopy of phase samples. ICOP & ICPET. 2017; 23 :741-744
URL: http://opsi.ir/article-1-1292-en.html

Volume 23 - Back to browse issues page
انجمن اپتیک و فوتونیک ایران Optics and Photonics Society of Iran
Persian site map - English site map - Created in 0.05 seconds with 31 queries by YEKTAWEB 3858