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Volume 25 - ICOP & ICPET 2019
ICOP & ICPET _ INPC _ ICOFS 2019, 25 - ICOP & ICPET 2019: 521-524 |
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Najafian H, Rashidian vaziri M R, Sajad B, Beygzadeh A, Abbasi A. Electric Field Determining the Size of Sephadex Biological Samples Using Digital holographic Microscopy Technique . ICOP & ICPET _ INPC _ ICOFS 2019; 25 :521-524
URL: http://opsi.ir/article-1-1870-en.html
URL: http://opsi.ir/article-1-1870-en.html
Hoorisa Najafian * 
1, Mohammad reza Rashidian vaziri2 , Batool Sajad1 , Amirmohammad Beygzadeh2 , Amir Abbasi3
1, Mohammad reza Rashidian vaziri2 , Batool Sajad1 , Amirmohammad Beygzadeh2 , Amir Abbasi3
1- Department of Physics, Alzahra University, Tehran, Iran
2- Radiation Application Research School, Nuclear Science and Technology Research Institute, Tehran
3- Photonics and Quantum Technologies Research School, Nuclear Science and Technology Research Institute, Tehran
2- Radiation Application Research School, Nuclear Science and Technology Research Institute, Tehran
3- Photonics and Quantum Technologies Research School, Nuclear Science and Technology Research Institute, Tehran
Abstract: (1522 Views)
In this study, size of a highly used biological substance in medicine called sephadex is measured using the digital microscopic interferometry technique. Initially, magnification of the designed interferometer was determined using standard diffraction gratings and then the recorded size of the diffraction gratings was used as a scale for measuring the size of the sephadex specimens. Standard diffraction gratings with 100, 300 and 600 lines per millimeter were used for calibrating the digital holographic microscopy setup. Diameter of a G200 sephadex particle is estimated about 123.8 micrometers and it is shown that the measurement is consistent with a separate measurement with neobar lam technique.
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