Optics and Photonics Society of Iran

This research has been conducted with goal to hemoglobin spectroscopy with non-invasive skin diffuse reflectance spectroscopy in compare to blood sample spectroscopy. For this, five normal cases in similar physiological and testing condition, was included. Two reflectance spectra with and without clearing from distal finger was acquired and then blood samples was tested invasively and the blood sample spectra were tested in transmission optical spectroscopy. Analytical quantification was run and it was found that the optical absorption coefficient in both non-invasive skin spectroscopy and blood sample optical spectroscopy were approximately equal. This might be beneficial to substitute non-invasive methods instead of invasive blood tests.