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Volume 25 - ICOP & ICPET 2019
ICOP & ICPET _ INPC _ ICOFS 2019, 25 - ICOP & ICPET 2019: 145-148 |
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Chartab jabbari M, Baradaran ghasemi A H, Latifi H. Point Spread Function reconstruction of an optical microscope
using large object imaging
. ICOP & ICPET _ INPC _ ICOFS 2019; 25 :145-148
URL: http://opsi.ir/article-1-1660-en.html
URL: http://opsi.ir/article-1-1660-en.html
1- Department of Physics, Shahid Beheshti University, Tehran, Iran
Abstract: (2194 Views)
In this paper, by using expectation–maximization for maximum likelihood estimation algorithm and by using spherical beads with two different sizes, the point spread function (PSF) is calculated in two different optical setups. The purpose of this experiment is to investigate the possibility of PSF reconstruction in an optical microscope through very large objects compared to that of the diffraction limit in the optical systems. We show that the PSF of an optical microscope can be estimated even through objects with size at least two times larger than the diffraction limit and with a noisy image.
Keywords: Point spread function, PSF, optical microscope, maximum likelihood estimation algorithm, Polystyrene beads
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