In this paper we propose an approach to determine the refractive index structure constant of turbulent atmosphere using the high frame rate imaging. This method is based on image jitter at the focal plane of the optical system. To determine the refractive index structure constant of environment, intensity variance of edge pixels, that have high intensity gradient, is used. Optical system parameters are determined regarded by the angle of arrival spatial and temporal correlation scale. This method has been examined experimentally, by using the captured images taken from the environment. Experimental results show good consistence with other methods.