Optics and Photonics Society of Iran

In this work, we have studied light-sheet microscopy using non-diffracting beam such as Bessel, and one- and two-dimensional Airy beams through numerical simulations. We have compared the effect of illumination by non-diffracting beams and a Gaussian beam. Our results reveal that the Bessel beams though provide microscopic images with lowest ghost due to scattering, suffer from a very low contrast due to the ring structure of the beam. On the other hand, scanned two-dimensional Airy beams are capable of providing microscopic images with a low ghost and a high contrast. Furthermore, we have also shown that that scanned two-dimensional Airy and scanned Bessel beams have a wider field of view and depth of focus comparing to the other beams. Due to these aspects, we conclude that scanned two-dimensional Airy and Bessel beams are very promising for utilizing in light-sheet microscopy systems