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Volume 26 - ICOP & ICPET 2020
ICOP & ICPET _ INPC _ ICOFS 2020, 26 - ICOP & ICPET 2020: 641-644 |
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Kafian H, Abbasian V, Akbari Birgani S, Moradi A, Abdollahpour D. Resolution enhancement of light-sheet fluorescence microscopy using a microsphere. ICOP & ICPET _ INPC _ ICOFS 2020; 26 :641-644
URL: http://opsi.ir/article-1-2141-en.html
URL: http://opsi.ir/article-1-2141-en.html
Hosein Kafian * 
1, Vahid Abbasian1 , Shiva Akbari Birgani1 , Ali-Reza Moradi1 , Daryoush Abdollahpour1
1, Vahid Abbasian1 , Shiva Akbari Birgani1 , Ali-Reza Moradi1 , Daryoush Abdollahpour1
1- Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan
Abstract: (1428 Views)
Light-sheet fluorescence microscopy (LSFM) is a powerful method 3D imaging of biological samples with the minimal photodamage, and high imaging speeds. However, achieving high lateral resolution for single cell imaging applications is a challenging issue for the method. Here, we demonstrate that using a transparent microsphere (250 µm diameter) in the imaging system, leads to 3.2x increase of the magnification, and an enhanced resolution. The capability of the approach is verified single-cell imaging of breast cancer cell line MCF7, and the results are compared with conventional LSFM images without the microsphere. Our proposed arrangement can be considered as an inexpensive and simple alternative for using high-NA objectives in LSFM.
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