1- Graduate university of advanced technology
2- Shahid Bahonar university of Kerman
2- Shahid Bahonar university of Kerman
Abstract: (2203 Views)
Fluorescence anisotropy is a measure of the polarization of fluorescence radiation of the molecule. This quantity provides valuable information on the structure and dynamics of the molecule and the effect of its surrounding environment. In this work, fluorescence anisotropy of methylene blue has been measured. Methylene blue is considered as a drug, as a photosensitizer and as a dye for staining cells and tissues. Two latter applications involve the interaction with light, so optical properties of the methylene blue including its emission anisotropy find theoretical and experimental significance. An optical setup was designed and implemented. The anisotropy of methylene blue in two different solvents with different viscosities was measured. The excitation wavelength dependency was investigated, too.
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |