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Azizi N, Najafloo A, Awalin Chaharsooghi M, Kowsar R, Masoumi R, Ahadi Akhlaghi E. Three-dimensional tracking of bovine sperm by digital holographic microscopy. ICOP & ICPET _ INPC _ ICOFS 2018; 24 :765-768
URL: http://opsi.ir/article-1-1542-en.html
URL: http://opsi.ir/article-1-1542-en.html
Negar Azizi * 
1, Akram Najafloo1 , Mohammad Awalin Chaharsooghi1 , Rasoul Kowsar2 , Reza Masoumi3 , Ehsan Ahadi Akhlaghi1
1, Akram Najafloo1 , Mohammad Awalin Chaharsooghi1 , Rasoul Kowsar2 , Reza Masoumi3 , Ehsan Ahadi Akhlaghi1
1- Department of Physics, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, 45137-66731, Iran
2- Department of Animal Science, Faculty of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran
3- Department of Animal Science, Faculty of Agriculture, University of Zanjan, Zanjan, 45371-38791, Iran
2- Department of Animal Science, Faculty of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran
3- Department of Animal Science, Faculty of Agriculture, University of Zanjan, Zanjan, 45371-38791, Iran
Abstract: (1854 Views)
Finding dynamical characteristics of particles has an especial importance in the area of soft and biological physics. This work introduces an experimental method to track bovine sperm in three dimension as movable bio particles by applying digital holographic microscopy. The work is based on applying image-processing methods and using the obtained information from the hologram record. Pixel size and Resolution of this microscope measured 175 nanometer and better than 2.19 micrometer respectively. We found the coordinates of the particles in three dimension. The average speed of the samples measured 127.51 micrometer per second.
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