Vesicles or artificial membranes are very similar to the cell membrane in size and structure, so it provides a good condition to study membrane’s response in presence of external agents in the scale of real cells. Optical tweezers is a well-known technique which is used to study cell's membrane. Usually, in these studies, dielectric micrometric particles are used as a handle. However, for intracellular study, it is better to use nanometric particles. Metallic nanoparticles are a good biocompatible candidate to be used for intracellular manipulation because of their size and chemical properties. Usually, in the process of vesicle preparation, nanometric vesicles are made and they are not clearly visible in the usual optical microscopy method. In this article to measure the force needed to inject a gold nanoparticle into a vesicle membrane, at the first, we used fluorescence microscopy to observe nanovesicles. Then, we trapped a 100 nm Au nanoparticle and is entered into the vesicle to measure the force.

Keywords: Fluorescent microscopy, Optical tweezers, Vesicle, Trapped metallic nanoparticles

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