In this study, for detection of Foeniculum vulgare essential oil spectrum, surface enhanced Raman scattering (SERS) spectroscopy has been used. Therefore, multiwalled carbon nanotubes/gold nanoparticles (MWCNTs/Au NPs) has been used as substrate for enhanced Raman scattering of essential oil extracted from Foeniculum vulgare seeds. Raman spectra of Foeniculum vulgare sample recorded and its peaks were assigned with respect to the molecular structure. Comparison of SERS and Raman spectra recorded with the spontaneous Raman scattering method, show the increase in the intensity of SERS spectra and confirms successful selection of SERS substrate.