In this paper the surface- enhanced Raman spectroscopy (SERS) of blood serum is reported which was done with drop coating deposition technique. The results show that not only the fluorescence background is decreased significantly but (also) the intensity of Raman peaks is enhanced by the factor of 30 that shows the capability of this method in Raman spectroscopy of biological samples in particular the biofluids. This technique can be used in studying the concentration of blood serum different contents.